When is pour plate used

Are you sure you want to delete your Profile? This will remove all of your posts, saved information and delete your account. This cannot be undone. Cancel Delete My Profile. Public Site Members. Cancel Share. Written by Sourav Bio. Best Books For Microbial Metabolism. Top 14 books for Industrial Microbiology. Top 15 Books for Food and diary Microbiology. Top 18 books for Environmental Microbiology. Reply Microbiology Techniques.

See also Standard technique: Making serial dilutions. To avoid contamination ensure: i that the water in the water bath is at the right depth ii that the bottles are kept upright iii that the outsides of the bottles are wiped before they are used.

At all times, hold the pipette as still as possible. Do not squeeze the teat bulb of the pipette after it is in the broth as this could cause bubbles and possibly aerosols. Gently release the required volume of inoculum onto the centre of the dish. Replace the lid. Like this: Like Loading Published by Ambali Adekunle. Previous post Nutrient Agar: composition, preparation and uses.

Next post What is Dengue Virus? There is certainly a lot to find out about this issue. I love all of the points you made. Leave a Reply Cancel reply Enter your comment here Fill in your details below or click an icon to log in:. Email required Address never made public. Name required. Create your website with WordPress.

Follow Following. Last updated on June 17th, CLED cysteine-, lactose-, and electrolyte-deficient agar is a differential culture medium primarily used for isolation and enumeration of bacteria especially from urine samples. CLED is preferred over a combination of […]. Last updated on June 21st, Microorganisms can produce different types of volatile compounds that may give characteristics smell, pleasant scent, or pungent odor.

Production of these volatile chemicals depends on the metabolic characteristics of […]. Last updated on June 3rd, Streak plate technique is used for the isolation into a pure culture of the organisms mostly bacteria , from a mixed population.

The inoculum is streaked over the agar surface […]. Quite helpful…!!! M in fy level now…will b helpful to me? Can you please tell me , why there is white patches occcur in plate count agar even when no microbial growth white patches still form on the plates. Why this happen with plate count agar?

During pour plate method, spreader colony found during total viable count then how to overcome this problem and which precautions take care during analysis. Does flaming the top of the agar jar add any benefit if performing testing in a laminar flow hood?