Why is dinitrophenylhydrazine used in transamination

Tryptophan transamination by purified pig AST was compared with that catalysed by preparations obtained from mammalian tissues. Unable to display preview. Download preview PDF. Skip to main content. This service is more advanced with JavaScript available. Advertisement Hide. Tryptophan metabolism via transamination. In vitro aminotransferase assay using dinitrophenylhydrazine method. This process is experimental and the keywords may be updated as the learning algorithm improves. This is a preview of subscription content, log in to check access.

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Bacciottini, D. Pellegrini-Giampietro, F. Bongianni, G. Beni, V. Politi, F. Moroni, Biochemical and behavioural studies on indole-pyruvic acid: a keto-analogue of tryptophan Pharmacol. Chung, G. Anderson, G. Fulk, Formation of indoleacetic acid by intestinal anaerobes J. This is favourable for resolution from other interfering hydrazones. Suitable conditions for tryptophan transamination in tissue and enzyme preparations were found.

Reaching optimal conditions for tryptophan transamination measurements in vitro is generally limited by low solubility of the amino acid in water solutions: With AST preparation, the velocity of catalysed reaction at x 10 -3 M tryptophan concentration was of 1st order to the amino acid substrate. Therefore the enzyme activity measurement at two different tryptophan concentrations is recommended for unknown samples.

Tryptophan transamination by purified pig AST was compared with that catalysed by preparations obtained from mammalian tissues.